Postdoctoral position on applying CRISPR/Cas9 technology in vivo


Department of Pediatrics, division of Molecular Genetics

Working Enviroment

A highly motivated individual with an extensive doctoral training in mouse genetics is being sought for a three year postdoctoral position in the laboratories of Alain de Bruin and Bart van de Sluis at the Department of Pediatrics, in the Division of Molecular Genetics at University Medical Center Groningen, in the Netherlands.

We have extensive expertise in generating novel mouse models. In addition we have unique capabilities to perform molecular, metabolic and pathological analysis of the newly generated mouse models. The powerful combination of generation in vivo models and applying state-of-the art analyzing technologies have been successfully used to study the Rb/E2F pathway controlling the cell cycle and its functional impact on development, aging, cancer and metabolism. Our efforts, a breakthrough in understanding the specific requirements of Rb and E2Fs in development, polyploidization and cancer, have been published in Nature 2003, Developmental Cell 2008, EMBO 2012, Nature Cell Biology 2012, Oncogene 2016 and Journal of Clinical Investigation 2016. More information about Prof. Dr. A. de Bruin, who has a joint position at Utrecht University and University Medical Center Groningen, are available on his website

Job description

The postdoc project will be focus on developing novel mouse models to study the role of the Rb/E2F pathway in liver ageing, metabolism, polyploidization and cancer. The postdoctoral researcher will generate novel knock-in mice by generating constructs to introduce mutations in members of the Rb/E2F pathway. In addition he/she will develop in vivo screening systems to inactivate and overexpress proteins of the Rb/E2F pathway in the mouse liver by using CRISPR/CAS technology in combination with adeno-associated viral delivery systems. The generation of the mouse models will be performed in the transgenic mouse facility headed by Bart van de Sluis. The postdoctoral researcher will be also responsible for analyzing the newly generated mouse models and will be supported by Dutch Molecular Pathology Center composed of veterinary pathologists and histo-technicians headed by Alain de Bruin. The impact of Rb/E2F pathway members on liver metabolism will be performed in collaboration with members of the Department of Pediatrics who have unique expertise for analyzing metabolic pathways.


Experiences with transgenic mouse models are required. Strong English speaking and writing skills are absolute essentials, as well as a track record of first author publications with an impact factor above 5. Strong problem-solving skills, creative thinking, and the ability to work independently are important.

Conditions of employment

You will have the opportunity to work on cutting-edge research, network with internationally renowned scientists and enjoy the benefits of joining a supportive researcher community. The aim of the program is to prepare researchers for careers as independent academic scientists.

Your salary will be a maximum of € 4.152,- gross per month (scale 10), depending on your qualifications and relevant experience, based on a full-time appointment. In addition, the UMCG will offer you 8% holiday pay, an 8.3% end-of-year bonus and a development budget. The terms of employment comply with the Collective Labour Agreement for Medical Centers (CAO-UMC).

More information
For more information about this vacancy you may contact:
Prof. Dr. A. de Bruin, email:
Prof. B. van de Sluis,

Applications should be accompanied by a letter of intend, full curriculum vitae, and list of references.

Additional information

Extra information can be obtained thru the following links:
Staff Veterinary Medicine
UMCG Corporate information/research
Welkome to Groningen

Applying for a job

Please use the the digital application form at the bottom of this page - only these will be processed. You can apply until 19 March 2017. Immediately after sending the digital application form you will receive an email- confirmation with further information.

Digital application form