PhD student and Postdoc on stem cell biology

Department

The European Research Institute for the Biology of Ageing (ERIBA)

Working Enviroment

ERIBA (The European Research Institute for the Biology of Ageing) is a newly established institute at the University Medical Centre Groningen (UMCG). The mission of ERIBA is to better understand the molecular mechanisms that result in age-related decline and age-related diseases. We aim to develop novel strategies to prevent or combat age-related diseases and to provide evidence-based recommendations for healthy ageing. The research teams are working with different model systems and technology platforms and share their knowledge and expertise to accelerate discoveries. The ERIBA is housed in a new building and is part of the central UMCG campus in the vibrant University City Groningen in the north of the Netherlands. Groningen is a small (200,000 inhabitants), but vibrant city hosting over 25,000 students.

Job description

Stem cells act in developing and adult organisms to produce the proper number of specialized cells in the body. It is important that stem cells select an appropriate division mode to ensure proper development, maintenance and repair of tissues. In the Paridaen lab, we aim to understand the mechanisms underlying stem cell decisions in developing and ageing tissues. We employ single cell analyses techniques to study stem cell behaviour in the developing brain, using zebrafish and mouse as our model systems. In particular, we are interested in the role of fate-determining factors such as signalling pathways and subcellular structures in symmetric and asymmetric stem cell divisions.

The Paridaen lab at ERIBA is currently looking for a highly motivated PhD student and post-doctoral researcher.

Phd student:
In the project funded by a NWO-Vidi grant, you will combine single cell analyses techniques with lineage tracing of neural stem cells in developing zebrafish. The overall project aim is to assess how certain cell biological and molecular properties of individual stem cells affect their sequential decisions on division modes and daughter cell fate specification. Techniques used include state-of-the-art live imaging technology, advanced tools for visualising the temporal dynamics of signalling activity, and spatiotemporally controlled methods of targeted manipulations such as optogenetics. Computational (dynamic system) modelling will be used in analysis of the results in collaboration with researchers at the Helmholtz Zentrum in Munich.

Qualifications:
- M.Sc. in bio-molecular sciences, medical biology or related area
- Inquisitive and independent research attitude
- Excellent communication skills and a team player
- Affinity or previous experience with computational (dynamic system) modelling and image analysis
- Experience with zebrafish model and certification to work with experimental animals is a big plus

Post-doctoral researcher:
In this project funded by a NWO-Vidi grant, you will investigate whether and which differences in gene expression dynamics underlie division outcomes at the single-cell level in mice and zebrafish. Techniques used include state-of-the-art live imaging technology, single-cell isolation and genomics, and targeted manipulations using CRISPR/Cas9. Single cell genomics will be performed in close cooperation with the experimental group of Prof. Dr. Peter Lansdorp. Computational modelling and bioinformatics will be used in analysis of the results in collaboration with colleagues in ERIBA and at the Helmholtz Zentrum in Munich.

Qualifications:
- PhD in bio-molecular sciences, medical biology or related area
- Inquisitive and independent research attitude
- Excellent communication skills and demonstrable ability to function in a team
- Experience with zebrafish or mouse model system, and certification to work with experimental animals
- Experience with next generation sequencing data and bioinformatics is a big plus
- Experience with CRISPR/Cas9–mediated genomic modification is a big plus

Requirements

Conditions of employment

The duration of the PhD project is four years to be concluded with a PhD examination. It concerns a full time appointment (36 h/week). After one year, the performance of the candidate will be evaluated to decide whether there is sufficient progress to expect a successful completion of the PhD thesis. Your salary is € 2.266,- gross per month in the first year up to a maximum of € 2.903,- gross per month in the last (fourth) year (scale PhD).

The duration of the Post-doc position is one year with the possibility for extension on a 0.8 fte appointment (28.8 h/week). Your salary will be a maximum of € 4.194,- gross per month (scale 10), depending on your qualifications and relevant experience, based on a full-time appointment.

In addition, the UMCG will offer you 8% holiday pay, an 8.3% end-of-year bonus and a development budget. The terms of employment comply with the Collective Labour Agreement for Medical Centers (CAO-UMC).

The UMCG has a preventive Hepatitis B policy. You may be required to build up sufficient protection against Hepatitis B before you can be appointed. Vaccination is provided by the UMCG if necessary.

How to apply
Please use the the digital application form at the bottom of this page - only these will be processed. You can apply until 3 September 2017.
Immediately after sending the digital application form you will receive an email- confirmation with further information.
Full applications including a resume, a motivation letter and the names of two references will receive full consideration.

Additional information

For more information about this vacancy you may contact:

Dr. J. Paridaen, Team leader, ERIBA; email: j.t.m.l.paridaen@umcg.nl (please do not use emailadress for applications)

Extra information can be obtained thru the following links:
Information about the Paridaen lab
Information about ERIBA
Welcome to Groningen

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